Fig. 4.

Extracting dermal components from a BCC skin section. In situ collagen (a) and elastin (b) are resolved from the image. The dye used by the dermatologist to mark the orientation of the tissue was also detected (c). Raman images are compared with the bright-field image (d), CLSM image (e) and histopathology image (f). The box on (e) marks the location of Raman imaging. The arrow in (f) points to a thin blue-gray elastic fiber. Plots on the right displays Raman spectrum of in situ collagen, synthetic collagen and the difference spectrum. Also Raman spectrum of in situ elastin, synthetic elastin, and their difference spectrum. Scale bar: 10 μm.