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(a)-(c) The images of three constituents, (a) Alexa 532, (b) Alexa 555 and (c) autofluorescence, separated by applying linear unmixing. (d) The combination image of (a) to (c). (e),(f) The linear unmixing results of spectra (blue lines) at (e) position A and (f) position B indicated in (d). Red lines: Alexa 532; green lines: Alexa 555; magenta lines: reconstructed spectra.
