Fig. 1.

CUBIC- and BABB-clearing in cardiac mouse tissue. A, Adapted CUBIC-clearing protocol for lectin-perfusion and clearing in mouse hearts. B, Stepwise enhancement of the sample transparency following the CUBIC-clearing protocol. C, Box-plot of the transparency measurement at different time points of the clearing protocol and at the end of BABB-clearing. D, Transparency measurement with Beer-Lambert law. I₀, initial light intensity; I, light intensity after traversing the uncleared/cleared tissue; L, tissue thickness; µ, absorption coefficient. E, F, Weight (E) and volume (F) measurements of the mouse hearts before, during and after the CUBIC- and BABB-clearing protocol. d, days in R1. n = 3. *p<0.05, **p<0.005, ***<0.0005, unpaired t-test vs control samples.