Fig. 3.

Illumination correction of SPIM images. A, Schematic representing the multiplication of the original image. The intensity loss in y (left) with the correction factor matrix (middle) leads to the illumination-corrected image (right). B, Intensity profiles along the yellow lines shown in the images in A. Blue, Intensity profile along the yellow line of the original SPIM image where the intensity loss along the light path can be observed. Red, Intensity profile of the N4-algorithm corrected image. Green, Intensity profile of the image that was corrected using the described algorithm implemented in Matlab. All images show the 405 nm laser excitation channel (DAPI), in which the highest Rayleigh scattering is present.