Fig. 1.

Sequence alignment of rsEGFP2 with EGFP and constructs design for BiFC assay. (a) Amino acids sequence alignment of rsEGFP2 (black) with EGFP(green), amino acids different from EGFP sequence are highlighted with dark red color. The 158Q splitting site of rsEGFP2 or EGFP for BiFC assay is indicated by red arrows. (b) β-Fos/β-Jun heterodimer model was used in establishing rsEGFP2-based BiFC assay. β-Jun was fused to the rsEGFP2-N with RSIAT linker while β-Fos or β-Fos(Δ Zip) was fused to the rsEGFP2-C with RPACKIPNDLKQKVMNH linker.