Fig. 5.

(a) Data simplex obtained in unsupervised unmixing of hyperspectral images of HeLa cells exposed to FCCP concentration (50-1000 µM). All images are analysed together. Gray points represent the image pixels, red squares represent the reference spectra, the location of vertices indicates the endmember spectra. The lines mark the sides of the simplex, the axes represent the top 2 PCA components; (b) The results of mean cellular abundance of free NAD(P)H show that FCCP quenches its fluorescence.(c) Mean cellular abundance of bound NADH also shows FCCP quenching. Data for all cells are represented by box plots [37, 38].