Fig. 1.

Aortic arch imaging. (A) A projection of the 3-D volumetric OCT data acquired for embryonic developmental staging. The image clearly displays the limb buds and wing buds indicated by the yellow arrows. These limb buds along with general head and body morphology are necessary for accurate staging without removal of the embryo from the yolk. (B) A phase variance image was generated from the Doppler OCT data to segment the aortic arch vessel lumen. The cross-sectional OCT images used to generate the phase variance image were acquired at the pharyngeal arches indicated by the dotted line in A. (C) The segmentation was applied to the Doppler OCT images for absolute blood flow calculations.