Fig. 4.

Pharyngeal arch mesenchymal tissue measurements. An orthogonal slice from a 3-D volumetric acquisition of the 3rd aortic arch of an uninjected (A) and ethanol-exposed (B) embryo. The lumen area was segmented as indicated by the yellow dotted line. The surrounding pharyngeal arch tissue was then segmented as indicated by the red dotted line. (C) Ethanol-exposed embryos (n = 19) did not have a statistically significant difference in the lumen cross sectional area compared with uninjected (n = 16) and saline (n = 17) embryos. (D) The surrounding pharyngeal arch tissue cross sectional area was statistically smaller in ethanol exposed embryos in comparison to uninjected and saline embryos. * indicates p<0.05.