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Optical metabolic imaging of BT474 2D monolayers. The OMI index was calculated at 24 (a), 48 (b), and 72 (c) hours post-drug treatment. Gold- and gray-colored bars represent changes versus control that are in agreement and disagreement, respectively, with prior 3D organoid studies [15]. Blue bars delineate the control group for comparison. Population density curves were also generated at 24 (d), 48 (e), and 72 (f) hour time points to resolve OMI index distributions across a population.
