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Comparison of fluorescence lifetime values in HR6 3D organoids and 2D monolayers. Average values for NAD(P)H (a) and FAD (b) τm in 2D monolayers and 3D organoids at 72 hours post-treatment reveal significantly greater values in 2D monolayers with respect to corresponding 3D organoid groups (p<0.001). Population density curves for NAD(P)H (c-f) and FAD (g-j) τm further distinguish 2D monolayer and 3D organoid models. The organoid data is a subset of data previously published in [15].
