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. 2017 Mar 15;22(6):686–695. doi: 10.1177/2472555217696794

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© 2017 Society for Laboratory Automation and Screening

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Functional expression in IonWorks Barracuda. Cells were thawed from cryogenic storage and dispensed into the assay plate in population patch clamp mode. (A) Current traces in plate view. (B) Sample records at higher magnification. The vertical line indicates application of ligand. The receptors were activated by application of either 0.3 or 3 µM (-)-nicotine. (C) Amplitude distribution. Holding potential, –70 mV. Mean seal resistance ± SD = 422.5 ± 84 MΩ (n = 380 wells). Four wells were invalid (<100 MΩ, shaded wells).