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. 2017 Mar 15;22(6):686–695. doi: 10.1177/2472555217696794

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© 2017 Society for Laboratory Automation and Screening

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Figure 2. — Agonist assay validation. (A) Concentration-response curves (16-point) were obtained for three full agonists in two independent experiments on different days. Compounds were administered at the indicated concentrations, and responses were recorded simultaneously. (B) Concentration-response curves (eight-point) for partial agonists, varenicline and cytisine. Data points represent mean peak current ± SEM (two to four replicate wells/concentration). Curves were fitted to the data by a one-site model.