Figure 4.

Subtype profiling of known and novel nicotinic acetylcholine receptors inhibitors. Mean IC₅₀ values ± SEM (n = 2–4) obtained in four-point (0.02–20 µM) or eight-point (methyllycaconitine only, 0.001–3 µM) concentration-response curves. The cells were preincubated with the test compounds for 2 min and then stimulated with (-)-nicotine at EC₉₀ concentrations (100 µM for α3β4, α4β2, and α3β4α5; 3 µM for α6/3β2β3^V273S; and 3 µM + 1 µM PNU 120596 for α7).