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Acceptance criteria.
| Parameter | Acceptance Criterion |
|---|---|
| PPC well level | |
| RSEAL (baseline)a | >100 MΩ |
| Current amplitude (baseline) | >0.1 nA |
| RSEAL stability (between first and second additions) | <50% decrease |
| PPC plate level | |
| Z′ factor | ≥0.4 |
| Success rate (% valid wells) | >90% accepted wells per PPC plate |
| EC/IC50 for reference compounds | ≤0.5 log from historical mean |
PPC = population patch clamp.
Typical Rseal values ranged between 200 and 1000 MΩ.
