TABLE 1.
Primer and probe sequences used in the standard and real-time Pneumoplex assays
| Organism and primer or probe | Sequence |
|---|---|
| B. pertussisa | |
| Forward primer | 5′-ATCGGGCATGCTTATGGGTGTTCA-3′ |
| Reverse primer | 5′-GCGGGCTAACTGTGAAGATTCAATAG-3′ |
| Probe | 5′-CTTGAGTGAACTGGGGGGTCGGCGATTTCCAGTT-3′ |
| C. pneumoniae | |
| Forward primer | 5′-AGGGCTATAAAGGCGTTGCT-3′ |
| Reverse primer | 5′-GTTAAGTTTAAAACAGCTGTA-3′ |
| Probe | 5′-GGAACAAAGTCTGCGACCATC-3′ |
| L. micdadei | |
| Forward primer | 5′-GTTGCTGACCGACGACCAGATGAAGGATGTGCTA-3′ |
| Reverse primer | 5′-TATTCCACAGTAACGACGTCGTCATCC-3′ |
| Probe | 5′-ATCCTTGAACGAGGCGATGGTGCTAAACCGACC-3′ |
| L. pneumophila | |
| Forward primer | 5′-CAATGTCAACAGCAATGGCTGCAAC-3′ |
| Reverse primer | 5′-CTCATAGCGTCTTGCATGCCTTTAGCC-3′ |
| Probe | 5′-CAAGGATAAGTTGTCTTATAGCATTGGTGCCGAT-3′ |
| M. pneumoniae | |
| Forward primer | 5′-GTTATGGAAACATAATGGAGGT-3′ |
| Reverse primer | 5′-TACAAGCTGGCGACTGTTTGTATTGGCCAT-3′ |
| Probe | 5′-TAACCGAGTGACAGGTGGTGCATGGTTGTCGT-3′ |
a
B. pertussis was tested only by the standard Pneumoplex assay (PCR-enzyme hybridization assay).