TABLE 5.
Organisms spiked in M4 mediuma | Dilution or TCID50s/mlb | OD at A450c |
---|---|---|
BP + CP | 106 + 104 | BP, >3.0; CP, >3.0 |
CP + LM | 104 + 106 | CP, >3.0; LM, >3.0 |
LM + LP | 106 + 106 | LM, >3.0; LP, >3.0 |
LP + MP | 106 + 106 | LP, >3.0; MP, >3.0 |
MP + BP | 106 + 106 | MP, >3.0; BP, >3.0 |
BP + CP | 107 + 104 | BP, >3.0; CP, >2.3 |
CP + LM | 103 + 106 | CP, >3.0; LM, 2.18 |
LM + LP | 107 + 106 | LM, 2.40; LP, >3.0 |
LP + MP | 107 + 107 | LP, >3.0; MP, 2.7 |
MP + BP | 107 + 107 | MP, 1.3; BP, >3.0 |
Abbreviations: BP, B. pertussis; CP, C. pneumoniae; LM, L. micdadei; LP, L. pneumophila; MP, M. pneumoniae.
The spiked concentrations of all organisms were based on the numbers of dilutions for all organisms except C. pneumoniae; for which the concentrations are TCID50s per milliliter.
ODs at A450 were determined on a spectrophotometer.