TABLE 2.
Summary of serological typing, DNA-DNA hybridization, porA sequencing, and Western blotting results for all meningococci identified with variations in the porA VR2-4 sequence
| Strain | Phenotype |
porA VR2 sequence
|
PorA expressed | Outcome of Western blottingd
|
|||
|---|---|---|---|---|---|---|---|
| DNAc | Amino acid | Epitope code | P1.7 | P1.4 | |||
| NZ99/38 | B:4:P1.4 | TATACGCCGGCTCATGTTGTTGTGAATAACAAGGTTGCTACTCACGTTCCGGCTGTTGTCGGC | HVVVNNKVATHVP | P1.4 | Yes | + | + |
| NZ96/142 | B:4:NSTa | TATACGCCGGCTCATGTTGTTGTGAATAACAAGGTTGCTACTCACGTTCCGGCTGTTGTCGGC | HVVVNNKVATHVP | P1.4 | Weak | − | − |
| NZ01/278 | B:4:NSTa | TATACGCCGGCTCATGTTGTTGTGAATAACAAGGTTGCTACTCACGTTCCGGCTGTTGTCGGC | HVVVNNKVATHVP | P1.4 | Weak | − | − |
| NZ02/55 | B:4:NSTa | TATACGCCGGCTCATGTTGTTGTGAATAACAAGGTTGCTACTCACGTTCCGGCTGTTGTCGGC | HVVVNNKVATHVP | P1.4 | Weak | − | − |
| NZ98/214 | B:4:NSTa | TATACGCCGGCTCATGTTGTTGTGAATAACCAGGTTGCTACTCACGTTCCGGCTGTTGTCGGC | HVVVNNQVATHVP | P1.4-8 | Yes | + | − |
| NZ01/56 | B:14:NSTa | TATACGCCGGCTCATGTTGTTGTGAATAACAAGGTTGCTCCTCACGTTCCGGCTGTTGTCGGC | HVVVNNKVAPHVP | P1.4-11 | Yes | + | + |
| NZ99/22 | B:4:NSTa | TATACGCC------------TGTGAATAACAAGGTTGCTACTCACGTTCCGGCTGTTGTCGGC | ---VNNKVATHVP | P1.4-9 | Yes | + | (+) |
| NZ02/68 | B:4:NSTb | TATACGCCGGCTCAT---------------------------------------GTTGTCGGC | HVV---------- | Deleted | Yes | + | − |
| NZ99/226 | B:4:NSTb | TATACGCCGGCTCAT---------------------------------------GTTGTCGGC | HVV---------- | Deleted | Weak | (+) | − |
| NZ97/27 | B:4:NSTb | TATACGCCGGCTCAT---------------------------------------GTTGTCGGC | HVV---------- | Deleted | Yes | + | (+) |
| NZ98/4 | B:4:NSTb | TATACGCCGGCTCATGTTGTTGTGAATAACAAGGTT---------------GCTGTTGTCGGC | HVVVNNKV----- | P1.4-5 | Yes | + | (+) |
| NZ03/43 | B:4:NSTb | TATACGCCGGCTCATGTTGTTGTGAATAACAAGGTTGCT------------GCTGTTGTCGGC | HVVVNNKVA---- | P1.4-5 | Yes | + | (+) |
| NZ94/124 | B:4:NSTb | TATACGCCGGCTCATGTTGTTGT------CAAGGTTGCTACTCACGTTCCGGCTGTTGTCGGC | HVVV--KVATHVP | P1.4-7 | Yes | + | (+) |
| NZ00/202 | B:4:NSTa | TATACGCCGGCTCATGTTGTTGT---TAACAAGGTTGCTACTCACGTTCCGGCTGTTGTCGGC | HVV-NNKVATHVP | P1.4-10 | Yes | + | − |
| NZ01/199 | B:4:NSTa | TATACGCCGGCTCATGTTGTTGT---TAACAAGGTTGCTACTCACGTTCCGGCTGTTGTCGGC | HVV-NNKVATHVP | P1.4-10 | Yes | + | (+) |
| NZ01/224 | B:4:NSTa | TATACGCCGGCTCATGTTGTTGT---TAACAAGGTTGCTACTCACGTTCCGGCTGTTGTCGGC | HVV-NNKVATHVP | P1.4-10 | Yes | + | (+) |
| NZ02/112 | B:4:NSTa | TATACGCCGGCTCAT---GTTGTGAATAACAAGGTTGCTACTCACGTTCCGGCTGTTGTCGGC | HVV-NNKVATHVP | P1.4-10 | Yes | + | (+) |
a
The porA P1.7 and P1.4 VR sequences identified by use of DNA-DNA hybridization.
b
The porA P1.7 VR sequence identified by use of DNA-DNA hybridization.
c
The region in boldface identifies the porA VR2 sequence.
d
(+), weak reaction.