Table 1.
Localization of identified proteins in the mitochondrial fraction of human embryonic stem cells (hESCs) according to MitoMiner 4.0 v2016 APR. MitoMiner reported mitochondrial localization of proteins based on subcellular immunofluorescent staining results from the Human Protein Atlas, large-scale mass-spectrometry, and GFP tagging data sets as well as computational predictions of mitochondrial targeting sequences from three popular mitochondrial target sequence prediction programs: iPSORT [5], TargetP [6], and MitoProt [7].
| Mitochondria localization evidence (percentage) | |
|---|---|
| Mito Evidence Mass-Spec Independent Studies | 70.5 |
| Mito Evidence GFP | 4 |
| Mito Evidence Mass-Spec Experiments | 70.5 |
| Mito Evidence Gene Ontology Annotation | 38.5 |
| Mito Evidence MitoCarta | 33.9 |
| Mito Evidence IMPI score (>0.7) | 39.9 |
| Mito Evidence Human Protein Atlas | 23.3 |
| Mito Targeting Seq iPSORT (>0.7) | 26.3 |
| Mito Targeting Seq MitoProt (>0.7) | 31.8 |
| Mito Targeting Seq TargetP (>0.7) | 21.5 |
| Mito Targeting Seq MitoFates (>0.7) | 18.4 |