Table 4. Coop(i) values for the terminal nucleotides in the P1 region^[a].

Nucleotide numbers	0 Mg2+		20 Mg2+
	Coop(i) ± SEM [b]	p [c]	Coop(i) ± SEM [b]	p [c]
15	0.009 ± 0.038	ns	0.065 ± 0.021	**
16	0.003 ± 0.036	ns	0.060 ± 0.019	**
17	-0.020 ± 0.036	ns	0.061 ± 0.017	***
18	-0.043 ± 0.037	ns	0.060 ± 0.016	***
19	-0.090 ± 0.040	ns	0.062 ± 0.015	***
77	-0.136 ± 0.039	**	0.062 ± 0.015	***
78	-0.043 ± 0.037	ns	0.060 ± 0.016	***
79	-0.020 ± 0.036	ns	0.061 ± 0.017	***
80	0.001 ± 0.036	ns	0.060 ± 0.019	**
81	0.005 ± 0.039	ns	0.068 ± 0.021	**

^[a] Coop(i) values were calculated based on Eq 2.

^[b] The SEM was calculated via error propagation from the single terms of Eq 2.

^[c] The p value was calculated using a two-sided one-sample t-test and the null hypothesis of equality to zero.

* p < 0.05;

** p < 0.01;

*** p < 0.001;

ns: p ≥ 0.05