Fig 1. Effects of Pseudomonas fluorescens strain 3JW1 on AFB₁ levels in PDB culture.

Aspergillus flavus was mixed with P. fluorescens strain 3JW1 in a 100-ml flask containing 15 ml of PDB. The final concentrations of A. flavus and P. fluorescens strain 3JW1 in PDB were 5×10⁵ spores/ml and 1×10⁷ CFU/ml, respectively. A mock control with PDB alone and another control inoculated with 1×10⁷ CFU/ml E. coli TOP10F′ were used. After 4 d (96 h) of incubation with constant shaking (200 r/min, 28°C), the medium filtrate was collected (except mycelium), and the amount of AFB₁ was determined by IAC-HPLC. This study was repeated 3 times, each with 3 replications.