Fig 2. Effects of Pseudomonas fluorescens strain 3JW1 on AFB₁ production in peanut medium.

Aspergillus flavus was mixed with P. fluorescens strain 3JW1 in a 100-ml flask containing 15 ml of peanut medium. The final concentrations of Aspergillus flavus and P. fluorescens strain 3JW1 in PDB were 5×10⁵ spores/ml and 1×10⁷ CFU/ml, respectively. A mock control with PDB alone and another control inoculated with 1×10⁷ CFU/ml E. coli were used. After 96 h of incubation with constant shaking (200 r/min) at 28°C in an incubator shaker, the medium filtrates were collected, and the amount of AFB₁ was determined by IAC-HPLC. This study was repeated 3 times, each with 3 replications.