Table 3. The treatment of living individuals of Siedleckia nematoides with cytoskeletal drugs.
| Changes / Time left after drug application | Drug / Concentration | |||||||
|---|---|---|---|---|---|---|---|---|
| Colchicine | Oryzalin | Jasplakinolide | Cytochalasin D | |||||
| 10 mM | 100 mM | 10 μM | 30 μM | 10 μM | 30 μM | 10 μM | 30 μM | |
| Initial increase of movement speed (0compared to control) | ≤ 10 min | ≤ 10 min | ≤ 20 min | ≤ 20 min | ≥ 5 min | ≥ 5 min | ≥ 30 min | ≥ 20 min |
| *0.55 ± 0.03 beats/s **1.86 ± 0.11 s | *0.56 ± 0.01 beats/s **1.78 ± 0.04 s | *0.57 ± 0.03 beats/s **1.77 ± 0.10 s | *0.59 ± 0.09 beats/s **1.85 ± 0.33 s | *0.59 ± 0.04 beats/s **1.70 ± 0.09 s | *0.54 ± 0.04 beats/s **1.99 ± 0.12 s | *0.61 ± 0.05 beats/s **1.68 ± 0.11 s | *0.59 ± 0.05 beats/s **1.79 ± 0.13 s | |
| Oscillating movement | + ≥ 10 min | + ≥ 10 min | + ≥ 20 min | + ≥ 20 min | - | - | - | - |
| First documented decrease of movement speed | ≤ 20 min | ≤ 15 min | ≥ 60 min | ≥ 45 min | ≥ 60 min | ≥ 30 min | ≥ 60 min | ≥ 120 min |
| Δ | Δ | *0.40 ± 0.05 beats/s **2.53 ± 0.32 s | *0.39 ± 0.04 beats/s **2.64 ± 0.28 s | *0.41 ± 0.02 beats/s **2.51± 0.12 s | *0.49 ± 0.06 beats/s **2.42 ± 0.24 s | *0.43 ± 0.03 beats/s **2.42 ± 0.20 s | *0.47 ± 0.04 beats/s **2.33 ± 0.23 s | |
| Progressive decrease of movement speed | ≥ 30 min | ≥ 20 min | ≥ 120 min | ≥ 60 min | ≥ 120 min | ≥ 60 min | ≥ 300 min | ≥ 240 min |
| *0.23 ± 0.03 beats/s **4.88 ± 0.65 s | *0.21 ± 0.02 beats/s **5.15 ± 0.46 s | *0.18 ± 0.02 beats/s **5.92 ± 0.60 s | *0.22 ± 0.04 beats/s **5.29 ± 0.69 s | *0.35 ± 0.04 beats/s **3.51 ± 0.72 s | *0.35 ± 0.04 beats/s **3.38 ± 0.61 s | *0.27 ± 0.05 beats/s **5.19 ± 0.82 s | *0.22 ± 0.01 beats/s **4.75 ± 0.27 s | |
| Spasmodic movement (bending) | - | - | + | + Restricted to anterior region | + | + Prevailing from side to side | + | + Prevailing to one side |
| Obvious cell rigidity (especially in posterior half) | + | + | + | + | - | - | - | - |
| Complete stoppage of motility | ≤ 120 min | ≤ 60 min | ≤ 480 min | ≤ 420 min | ≤ 480 min | ≤ 360 min | ≥ 540 min | ≤ 480 min |
| Recovery of motility in majority of blastogregarines after washing in seawater | ≤ 120 min | ≤ 90 min | ≥ 60 min | ≥ 60 min | ≥ 60 min | ≥ 60 min | ≥ 60 min | ≥ 60 min |
The symbol + indicates some observed change in the character of motility;—no obvious changes; ≥ changes appeared after the noted time period; ≤ changes appeared only during the noted time period
* beat frequency (beats/s = in Hz equivalent to 1 beat cycle per second, average ± standard error of the mean)
** beat to beat interval (an average time between the two beats ± standard error of the mean)
Δ beat frequency not measurable due to high sample variability.
0 Control individuals continued beating for more than 9 h (entire experiment duration); they beat at a rate of 0.51 ± 0.02 beats/s with beat to beat interval of 2.18 ± 0.13 s.