Figure 2.

Color-Coded H9M Cells Show Comparable Properties In Vivo

(A) Schematic experimental design. H9M cells were transduced in separate wells with either one of six FGB vectors, before sorting and transplantation of single color-coded populations per mouse. The PB was sampled over time, and the PB and BM status were assessed in moribund mice. (B) Determination of single color-coded cell fractions within donor-derived PB CD45.1⁺, CD45.1⁺CD11b⁺Gr1⁻, and CD45.1⁺CD11b⁺Gr1⁺ cells 3 and 5 weeks post-transplantation and at the time of sacrifice (sac). (C) Color code content in BM LSC subpopulations is shown. (D) Survival curves for all six single color code transplanted groups (4 mice per group). The number of mice analyzed per group and time point is indicated in Figures S5A and S5B. The bar graphs represent mean values ± SD.