Figure 3.

In Vivo Competition of Six Color-Coded H9M Populations Results in a Loss of Color Code Complexity over Time

(A) Experimental design. Purified color-coded cells were mixed in equal ratios and transplanted into lethally irradiated mice. The PB was sampled over time, and the PB and BM were analyzed in moribund mice. (B) Color code distribution within (left) donor-derived CD45⁺ PB cells over time, (middle) bulk BM cells, and (right) BM LSC subpopulations (subp.) of mice transplanted with 6xFGB transduced H9M (CD45.1 or CD45.2) cells. (C) Determination of color codes >10% contribution to the donor-derived PB cell fraction, from a total of 19 transplanted mice, 16, 18, 19, 12, and 18 were analyzed 3, 4, 5, and 6 weeks post-transplantation and at the time of sacrifice, respectively. The bar graphs from (C) represent mean values ± SD.