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. 2017 Jun 22;12(6):e0180096. doi: 10.1371/journal.pone.0180096

Fig 1. Schematic overview and FACS analysis of in vitro, murine Nkx2.5 EmGFP ES cell-based cardiac differentiation.

Fig 1

A) schematic representation of homologously recombineered, 2A self-cleaving EmGFP reporter inserted immediately downstream of the endogenous Nkx2.5 promoter and immediately upstream of the Nkx2.5 gene in mouse ES cells, B) overview of gene expression trends for in vitro cardiac-directed differentiation, sorting and purification of Nkx2.5 EmGFP reporter ES cells, C) FACS analysis of Nkx2.5 EmGFP ES cells at d6, d8 and d10 of cardiac differentiation, D) EmGFP gene expression kinetics for non-sorted Nkx2.5 EmGFP ES cells throughout cardiac differentiation (* p<0.05, n = 3).