Fig 5. Simulated hybrid assemblies: NGA50 against long-read depth.

Mean NGA50 values for hybrid assemblies of simulated read sets. Mean values were calculated across all read lengths, read accuracies and replicate tests for each reference genome (210 hybrid-read sets each); the top panel shows mean values for all 12 reference genomes (2520 hybrid-read sets). Horizontal dashed lines indicate the N50 size of the reference genome. For the bacterial genomes, this is the size of their only chromosome; for Saccharomyces, it is the size of chromosome XIII, an intermediate-sized replicon in the genome.