TABLE 2.
Gene | PCR grouping | Size of PCR product (kb)c | No. (%) of isolates (n = 106) | No. (%) of strains (n = 86)b |
---|---|---|---|---|
plcA | plcA-I | 1.6a | 102 (96.2) | 84 (97.6) |
plcA-II | 3.0 | 2 (1.9) | 1 (1.2) | |
plcA-III | Negative | 2 (1.9) | 1 (1.2) | |
plcB | plcB-I | 1.8a | 104 (98.1) | 85 (98.8) |
plcB-II | Negative | 2 (1.9) | 1 (1.2) | |
plcC | plcC-I | 1.8a | 102 (96.2) | 83 (96.5) |
plcC-II | 3.0 | 3 (2.8) | 3 (3.5) | |
plcC-III | Negative | 1 (0.9) | ||
plcD | plcD-I | 1.9a | 66 (62.3) | 50 (58.8) |
plcD-II | 3.2 | 19 (17.9) | 17 (20.0) | |
plcD-III | 2.7 | 1 (0.9) | 1 (1.2) | |
plcD-IV | 2.1 | 1 (0.9) | 1 (1.2) | |
plcD-V | Negative | 19 (17.9) | 16 (18.8) |
Size identical to that of the PCR products of the positive control strain CDC1551.
The frequency of each plcABC genotype among the strains was determined based on a total of 86 strains, as two isolates of one strain were found to have different plcABC genotypes. The frequency of each plcD genotype among the strains was calculated based on the data for 85 of the 87 strains, as two strains showed a plcD genotype different from that of the clustered isolates.
Negative, no PCR product.