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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Mar 29;1862(7):706–715. doi: 10.1016/j.bbalip.2017.03.011

Fig. 5. RP-HPLC analysis of products formed from 13S-HPOTE by Fg-cat.

Fig. 5

Products were chromatographed using a Waters Symmetry C18 column (0.46 × 25 cm), a solvent of CH3CN/water/glacial acetic acid (60:40:0.01 by volume) at a flow rate of 1 mL/min, with the UV profiles depicted at 235 nm (red) and 280 nm (black). The main peaks were further purified by SP-HPLC and the structures established by LC-MS and 1H-NMR (Supplement Fig. S3, Tables S3–S6).