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The recombinant 19-kDa protein is recognized by serum from infected cattle. Affinity purified MBP (lane 1) and MBP-19 kDa (lane 2) were transferred onto membranes in equal amounts and probed with sera from three clinically infected cattle: (A) animal 107; (B) animal 116; (C) animal 167. Reactivity was observed for the 19-kDa protein but not the MBP protein. Size standards are indicated in kilodaltons in the left margin.
