Table 4.

FISH analyses of the abundance of the Methanosaeta spp. and their association with the A6.

Measurement	AD location
	Ejby Mølle	Slagelse	Randers	Aalborg East
Archaea % biovolume∗	4 ± 2	2 ± 1	5 ± 1	2 ± 1
Methanosaeta % biovolume∗	2 ± 1	1 ± 1	3 ± 1	1 ± 1
% Methanosaeta with associated A6∗∗	23 ± 3	2 ± 1	1 ± 1	0

Values are averages ± standard deviation, calculated from ^∗25 image sets or ^∗∗3 manual counts of 100 randomly selected Methanosaeta filaments (>10 μm in length). Methanosaeta filaments were considered positive for association with A6 filaments if multiple of the latter appeared horizontally attached to the former. 12 other mesophilic ADs located in Denmark were screened but excluded from analyses due to high background fluorescence or the absence or low abundance of the A6.