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. 2017 Jun 22;7:4104. doi: 10.1038/s41598-017-04205-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overview of blue versus red light evoked activity in the brain. (a) Optical sections in a 6 dpf elavl3:GCaMP6f fish, going from dorsal to ventral. Each plane is separated by 10 µm. The area imaged at each plane is 443.47 µm x 222.73 µm. (b) Distribution of light-evoked activity in one fish, generated from ICA spatial maps. The white arrowheads indicate the thalamus, while the arrow indicates the habenula. (b) A magnified view of the area boxed in panel (b). (c–f) Different classes of responses, obtained by PCA followed by ICA. The same colours are used in panel (b). The blue and red bars indicate the periods that blue or red light was delivered. (g) Light-evoked activity in segmented ROIs, which were derived from ICA spatial maps. OT: optic tectum; Hb: habenula; a: anterior; p: posterior. Gamma of 0.65 was applied to panels (a and b). Scale bar = 50 µm.