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. 2017 Jun 22;7:4040. doi: 10.1038/s41598-017-04355-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CaMK2N1 shRNA expression in dorsal hippocampus does not affect contextual fear LTM formation, storage and retrieval. (a) Dorsal hippocampi of mice were transfected with rAAV expressing either control shRNA (n = 19) or CaMK2N1 shRNA (n = 20). Contextual fear LTM was determined 4 days after conditioning. (b) Quantitative analysis of CaMK2N1 mRNA expression in dorsal hippocampi of animals from (a) (shControl, n = 19; shCaMK2N1, n = 20). (c) Quantitative analysis of CaMK2N2 mRNA expression in dorsal hippocampi of animals from (a). (shControl, n = 19; shCaMK2N1, n = 20). (d) Freezing scores obtained during conditioning of the animals from (a). Training session is divided here between before and after the shock. No animal in the shCaMK2N1 group presented any freezing before shock, resulting in a mean freezing of 0 and 0 of S.E.M. Two-way ANOVA test with repeated measures revealed a significant effect of the foot-shock (F_1,37 = 32.0; P < 0.001), with no effect of rAAV treatment (F_1,37 = 1.7; P = 0.120) or interaction between these two factors (F_1,37 = 0.0; P = 0.84). SNK post-hoc comparisons revealed a significant increase in freezing scores between before and after the shock for both shControl (n = 19) (q = 5.7; P < 0.001) and shCaMK2N1 (n = 20) (q = 5.5; P < 0.001) groups. No significant difference was obtained when comparing animals from shControl and shCaMK2N1 within before (q = 1.0; P = 0.44) or after (q = 1.4; P = 0.29) foot-shock. Mean and S.E.M.; ***P < 0.001.