Figure 1.
The binding capacity of P. falciparum isolates IT4 and 3D7 to wild type CHO-745 cells and to various endothelial receptors before (−) and after (+) enrichment for binding to a receptor of interest. CHO-745 cells expressing the receptor of interest on their surface were used to analyse the binding capacity of isolates IT4 (A) and 3D7 (B). After incubation of trophozoite-stage IEs with the respective CHO-745 cell monolayer seeded on a coverslip, the slides were washed, fixed with 1% glutaraldehyde and finally stained with Giemsa stain. Bars represent the mean number ± SD of IEs that bound per 100 CHO-745 cells, as determined by light microscopic evaluation. CHO-745, non-transfected CHO-745 WT cells. Statistical significance was determined using the unpaired t-test; **p < 0.01, ***p < 0.001, ****p < 0.0001.