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. 2017 Jun 23;8:425. doi: 10.3389/fphys.2017.00425

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Copyright © 2017 Bartolák-Suki, Noble, Bou Jawde, Pillow and Suki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative western blots and normalized surfactant protein (SP) levels from lavage fluid and lung tissue as a function of ventilation mode as described in Figure 1. (A) Top: representative western blots for SP-B (~18 kDa) in the bronchoalveolar lavage (BAL), proSP-B (~40 kDa) from lung tissue, the dimer form of SP-B (~18 kDa) from lung tissue and the loading control (LC) β-actin (~42 kDa). Bottom: statistics show that SP-B in BAL increases with VV2 relative to VV1 (p < 0.005). The proSP-B is higher during VV2 than CV (p < 0.005) whereas the levels of the dimer are different for all pairwise comparisons (p < 0.001). (B) Top: representative western blots for proSP-C (~21 kDa) in BAL and lung tissue (~25 kDa) together with the β-actin LC (~42 kDa). Bottom: Compared to CV, proSP-C decreases with VV1 and VV2 both in the lavage (p < 0.001) and the lung (p = 0.006). (C) Top: representative western blots for SP-A (~38 kDa) in BAL and lung tissue (~26 kDa) together with the β-actin LC (~42 kDa). Bottom: Compared to CV, SP-A in the lavage decreases with VV2 (p < 0.001), and in the lung, it increases with VV1 and VV2 (p < 0.001). (D) Top: representative western blots for SP-D (~35 kDa) in BAL and lung tissue (~35 kDa) together with the β-actin LC (~42 kDa). Bottom: Compared to CV, SP-D decreases with VV1 and VV2 both in the lavage and the lung (p < 0.001) and there is also a significant difference between VV1 and VV2. ^* and # denote statistically significant difference from CV and VV1, respectively.