. 2017 Jun 23;8:1101. doi: 10.3389/fpls.2017.01101

Table 3.

Relative concentration and fold changed of differential metabolites among soja seedling roots after 14 days of alkaline salt stress.

Metabolite name		Relative concentration						Fold changes ${Log}_{2}^{(A S / C K)}$
		W		S		M
		CK	AS	CK	AS	CK	AS	W	S	M
Amino acids	Proline	0.19 ± 0.03	1.07 ± 0.13	0.47 ± 0.06	2.70 ± 0.71	0.82 ± 0.04	1.02 ± 0.21	2.60^**	2.53^*	0.32
	Phenylalanine	0.03 ± 0.00	0.08 ± 0.01	0.13 ± 0.01	0.23 ± 0.01	0.17 ± 0.01	0.16 ± 0.01	1.30	0.84^*	−0.08
	Glutamic acid	0.00 ± 0.00	0.05 ± 0.01	0.03 ± 0.01	0.07 ± 0.01	0.03 ± 0.01	0.05 ± 0.02	4.46^*	1.29^*	0.63
	Aspartic acid	0.64 ± 0.40	10.03 ± 0.81	2.35 ± 0.32	1.10 ± 0.90	0.86 ± 0.09	2.23 ± 0.15	3.97^**	0.78	1.38^*
	L-allothreonine	0.07 ± 0.03	0.23 ± 0.03	0.16 ± 0.01	0.34 ± 0.03	0.20 ± 0.02	0.25 ± 0.04	1.66^*	1.05^*	0.32
	Isoleucine	0.14 ± 0.04	0.69 ± 0.08	0.36 ± 0.04	1.56 ± 0.25	0.54 ± 0.01	1.16 ± 0.05	2.26^**	2.13^**	1.10^*
	Valine	0.50 ± 0.11	1.54 ± 0.13	0.75 ± 0.08	3.00 ± 0.48	1.04 ± 0.04	2.19 ± 0.07	1.62^**	1.99^**	1.08^*
	Glycine	0.81 ± 0.09	1.80 ± 0.10	0.55 ± 0.06	2.62 ± 0.43	0.74 ± 0.05	1.88 ± 0.37	1.15^**	2.25^**	1.35^*
	Serine	0.16 ± 0.03	0.65 ± 0.07	0.79 ± 0.07	1.00 ± 0.07	0.91 ± 0.02	0.95 ± 0.05	2.00^*	0.34	0.06
	Alanine	1.56 ± 0.30	5.15 ± 0.74	2.90 ± 0.26	6.65 ± 0.81	4.64 ± 0.58	5.91 ± 0.56	1.72^**	1.20^**	0.35
	Asparagine	0.03 ± 0.00	0.17 ± 0.04	0.07 ± 0.01	0.23 ± 0.02	0.19 ± 0.03	0.11 ± 0.01	2.49	1.63^**	−0.79
Fatty acids	Arachidic acid	0.06 ± 0.01	0.10 ± 0.01	0.05 ± 0.00	0.07 ± 0.01	0.04 ± 0.00	0.04 ± 0.01	0.66^*	0.38	−0.03
	Lignoceric acid	0.03 ± 0.00	0.04 ± 0.01	0.02 ± 0.00	0.03 ± 0.00	0.02 ± 0.00	0.02 ± 0.00	0.56	0.74^**	−0.26
	Behenic acid	0.03 ± 0.00	0.06 ± 0.00	0.03 ± 0.00	0.04 ± 0.00	0.03 ± 0.00	0.02 ± 0.00	0.88	0.48^**	−0.62^*
	Arachidonic acid	0.09 ± 0.01	0.10 ± 0.01	0.03 ± 0.00	0.06 ± 0.01	0.05 ± 0.00	0.04 ± 0.01	0.06	1.22^**	−0.26
	Oleic acid	0.00 ± 0.00	0.03 ± 0.00	0.01 ± 0.00	0.04 ± 0.00	0.01 ± 0.00	0.03 ± 0.00	3.17^**	1.90^**	1.15^**
	Elaidic acid	0.09 ± 0.01	0.13 ± 0.04	0.05 ± 0.00	0.07 ± 0.01	0.06 ± 0.01	0.07 ± 0.01	0.46	0.69^*	0.39
	Cis-gondoic acid	0.00 ± 0.00	0.03 ± 0.00	0.01 ± 0.00	0.03 ± 0.00	0.01 ± 0.00	0.03 ± 0.00	3.24^**	2.40^**	2.00^**
	D-glyceric acid	0.36 ± 0.02	0.41 ± 0.03	0.20 ± 0.01	0.09 ± 0.01	0.20 ± 0.02	0.09 ± 0.03	0.19	−1.20^*	−1.23^**
TCA	Succinic acid	0.19 ± 0.02	0.10 ± 0.01	0.09 ± 0.00	0.04 ± 0.01	0.10 ± 0.01	0.03 ± 0.00	−0.94^**	−1.06^**	−1.74^**
	Citramalic acid	0.05 ± 0.00	0.03 ± 0.00	0.02 ± 0.00	0.01 ± 0.00	0.04 ± 0.01	0.01 ± 0.00	−0.52^*	−0.90^**	−2.43^**
	Glucose-1-phosphate	0.20 ± 0.02	0.10 ± 0.02	0.19 ± 0.00	0.07 ± 0.01	0.17 ± 0.00	0.07 ± 0.01	−1.09^*	−1.42^**	−1.29^**
	Fumaric acid	2.10 ± 0.21	7.74 ± 0.93	0.71 ± 0.10	0.57 ± 0.35	0.95 ± 0.08	0.62 ± 0.18	1.88^**	−0.32	−0.61
	L-malic acid	26.68 ± 1.30	63.42 ± 6.58	11.16 ± 1.27	2.49 ± 0.52	13.45 ± 1.34	5.95 ± 1.85	1.25^*	−2.16^**	−1.18^*
	Citric acid	7.58 ± 1.00	29.38 ± 3.09	5.37 ± 1.19	7.40 ± 3.245	2.86 ± 0.41	3.85 ± 0.88	1.96^**	0.46	0.43
	Malonic acid	1.25 ± 0.18	3.93 ± 1.07	1.26 ± 0.22	1.11 ± 0.42	1.86 ± 0.22	0.49 ± 0.05	1.65^*	−0.18	−1.93^*
	Maleic acid	0.02 ± 0.01	0.04 ± 0.01	0.02 ± 0.00	0.01 ± 0.00	0.02 ± 0.00	0.01 ± 0.00	1.18	−0.63	−1.51^**
Carbohydrates and polyols	Mannitol	0.11 ± 0.02	0.03 ± 0.00	0.07 ± 0.01	0.02 ± 0.00	0.03 ± 0.00	0.01 ± 0.00	−1.83^*	−2.27^**	−1.80^*
	D-Arabitol	0.06 ± 0.00	0.04 ± 0.00	0.05 ± 0.00	0.03 ± 0.00	0.06 ± 0.01	0.02 ± 0.00	−0.54^**	−0.79^**	−1.79^**
	Dihydroxyacetone	0.05 ± 0.01	0.04 ± 0.01	0.03 ± 0.00	0.01 ± 0.00	0.05 ± 0.00	0.02 ± 0.00	−0.33	−1.27^**	−1.48^**
	Xylitol	2.27 ± 0.33	1.04 ± 0.09	0.94 ± 0.02	0.23 ± 0.05	1.05 ± 0.13	0.23 ± 0.05	−1.12^*	−2.02^**	−2.21^**
	Phytosphingosine	0.06 ± 0.00	0.03 ± 0.00	0.02 ± 0.00	0.01 ± 0.00	0.01 ± 0.00	0.01 ± 0.00	−0.85^*	−1.32^**	−0.04
	Maltotriose	0.05 ± 0.00	0.05 ± 0.00	0.04 ± 0.00	0.01 ± 0.00	0.03 ± 0.01	0.01 ± 0.00	−0.16	−1.67^**	−1.56^**
	Tagatose	18.06 ± 0.69	3.83 ± 0.53	8.76 ± 0.19	2.31 ± 0.63	10.10 ± 1.40	0.61 ± 0.34	−2.24^**	−1.92^**	−4.04^**
	Galactose	0.20 ± 0.01	0.18 ± 0.01	0.20 ± 0.01	0.09 ± 0.03	0.39 ± 0.02	0.09 ± 0.01	−0.18	−1.10^**	−1.95^**
	Glucose	0.07 ± 0.01	0.02 ± 0.00	0.03 ± 0.00	0.01 ± 0.00	0.03 ± 0.00	0.01 ± 0.00	−1.79^**	−1.35^**	−1.81^**
	Fucose	0.24 ± 0.03	0.10 ± 0.02	0.10 ± 0.01	0.03 ± 0.00	0.13 ± 0.02	0.02 ± 0.00	−1.27^**	−1.67^**	−2.68^**
	Ribose	7.28 ± 0.69	3.07 ± 0.49	2.75 ± 0.09	1.60 ± 0.30	4.36 ± 0.45	0.90 ± 0.19	−1.04^**	−0.78^**	−2.28^**
	Mannose	19.20 ± 0.63	0.34 ± 0.11	9.35 ± 0.27	1.13 ± 0.44	8.40 ± 0.71	0.32 ± 0.20	−5.82^**	−3.04^**	−4.73^**
	Fructose	23.60 ± 0.45	5.14 ± 0.37	11.56 ± 0.46	2.90 ± 1.47	14.25 ± 1.87	0.86 ± 0.47	−2.20^**	−2.00^**	−4.05^**
	Sucrose	0.28 ± 0.09	0.33 ± 0.11	0.42 ± 0.32	0.20 ± 0.19	7.21 ± 0.57	0.05 ± 0.03	0.23	−1.03	−7.25^**
Carboxylic acid	4-Aminobutyric acid	11.82 ± 0.96	3.47 ± 0.49	6.77 ± 0.49	2.00 ± 1.02	4.53 ± 0.22	1.21 ± 0.34	−1.77^**	−1.76^**	−1.90^**
	5-Aminovaleric acid	0.50 ± 0.07	0.34 ± 0.15	0.52 ± 0.07	0.08 ± 0.04	0.39 ± 0.12	0.06 ± 0.03	−0.55	−2.66^**	−2.72^*
	Galactonic acid	1.99 ± 0.53	1.43 ± 0.26	1.40 ± 0.21	0.56 ± 0.35	3.50 ± 0.37	0.32 ± 0.17	−0.48	−1.34	−3.47^**
	Saccharic acid	0.21 ± 0.02	0.16 ± 0.02	0.16 ± 0.00	0.06 ± 0.01	0.20 ± 0.02	0.05 ± 0.01	−0.44	−1.44^**	−1.94^**
	Threonic acid	12.03 ± 0.57	7.04 ± 0.31	4.75 ± 0.51	1.26 ± 0.13	5.80 ± 0.69	0.79 ± 0.13	−0.77^*	−1.91^*	−2.88^**
	4-Hydroxy-3-methoxybenzoic acid	0.03 ± 0.00	0.01 ± 0.00	0.05 ± 0.01	0.02 ± 0.00	0.06 ± 0.01	0.01 ± 0.00	−0.99^**	−1.50^*	−1.90^**
	Digalacturonic acid	0.03 ± 0.00	0.03 ± 0.00	0.02 ± 0.00	0.01 ± 0.00	0.01 ± 0.00	0.01 ± 0.00	0.05	−0.68^*	−0.01
	Glycolic acid	0.16 ± 0.03	0.25 ± 0.07	0.06 ± 0.01	0.04 ± 0.00	0.06 ± 0.01	0.04 ± 0.00	0.64	−0.80^**	−0.54^*
	Gluconic acid	10.20 ± 1.17	36.88 ± 0.80	7.27 ± 0.72	12.53 ± 1.94	20.29 ± 1.05	6.10 ± 1.24	1.85^**	0.78	−1.73^**
	Myristic Acid	0.05 ± 0.00	0.06 ± 0.01	0.04 ± 0.00	0.05 ± 0.01	0.03 ± 0.00	0.05 ± 0.00	0.38	0.58	0.55^*
Nucleic acid	Thymidine	0.01 ± 0.00	0.01 ± 0.00	0.01 ± 0.00	0.02 ± 0.00	0.00 ± 0.00	0.01 ± 0.00	0.23	1.49^**	1.66^**
	Thymine	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.03 ± 0.00	0.01 ± 0.00	0.02 ± 0.00	−0.47	4.28^**	1.15^**
	Uracil	0.52 ± 0.04	0.73 ± 0.06	0.48 ± 0.02	0.75 ± 0.03	0.51 ± 0.02	0.59 ± 0.04	0.48	0.66^**	0.20
ROC	Tricetin	0.02 ± 0.00	0.02 ± 0.00	0.01 ± 0.00	0.01 ± 0.00	0.01 ± 0.00	0.01 ± 0.00	0.11	0.00	−0.96^**
	5-Methoxytryptamine	0.00 ± 0.00	0.18 ± 0.10	0.06 ± 0.00	0.01 ± 0.00	0.10 ± 0.00	0.03 ± 0.00	5.92^**	−2.13^**	−1.54^**
	Sitosterol	0.02 ± 0.00	0.02 ± 0.00	0.01 ± 0.00	0.01 ± 0.00	0.02 ± 0.00	0.01 ± 0.00	−0.05	0.92^**	−0.67
	Salicylic acid	0.02 ± 0.00	0.02 ± 0.00	0.01 ± 0.00	0.01 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.42^*	−0.63^*	−2.09^**
	Naringin	0.02 ± 0.00	0.02 ± 0.00	0.00 ± 0.00	0.02 ± 0.00	0.01 ± 0.00	0.00 ± 0.00	−0.07	3.93^**	−0.64^**
	Hydroxylamine	0.08 ± 0.00	0.05 ± 0.00	0.02 ± 0.00	0.02 ± 0.01	0.02 ± 0.01	0.01 ± 0.00	−0.80^**	0.13	−0.71
	Gallic acid	0.49 ± 0.05	0.58 ± 0.06	0.44 ± 0.02	0.38 ± 0.04	0.14 ± 0.01	0.33 ± 0.02	0.24	−0.21	1.19^**
	Fluorene	0.00 ± 0.00	0.06 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.02 ± 0.00	0.01 ± 0.01	4.29^**	1.58^*	−1.15

The relative contents of metabolites are the means of data from four biological replicates using GC–MS. The fold changes was calculated using the formula ${log}_{2}^{(s a l t / c o n t r o l)}$ . Values were presented as the mean ± standard deviation of four biological replicates. CK, control treatment; NS, neutral salt stress; AS, alkaline salt stress. W, wild soybean; S, semi-wild soybean; M, cultivated soybean.

Significant difference at p < 0.05.

^**

Significant difference at p < 0.01.