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. 2017 Jun 23;8:1101. doi: 10.3389/fpls.2017.01101

Table 3.

Relative concentration and fold changed of differential metabolites among soja seedling roots after 14 days of alkaline salt stress.

Metabolite name Relative concentration Fold changesLog2(AS/CK)
W S M
CK AS CK AS CK AS W S M
Amino acids Proline 0.19 ± 0.03 1.07 ± 0.13 0.47 ± 0.06 2.70 ± 0.71 0.82 ± 0.04 1.02 ± 0.21 2.60** 2.53* 0.32
Phenylalanine 0.03 ± 0.00 0.08 ± 0.01 0.13 ± 0.01 0.23 ± 0.01 0.17 ± 0.01 0.16 ± 0.01 1.30 0.84* −0.08
Glutamic acid 0.00 ± 0.00 0.05 ± 0.01 0.03 ± 0.01 0.07 ± 0.01 0.03 ± 0.01 0.05 ± 0.02 4.46* 1.29* 0.63
Aspartic acid 0.64 ± 0.40 10.03 ± 0.81 2.35 ± 0.32 1.10 ± 0.90 0.86 ± 0.09 2.23 ± 0.15 3.97** 0.78 1.38*
L-allothreonine 0.07 ± 0.03 0.23 ± 0.03 0.16 ± 0.01 0.34 ± 0.03 0.20 ± 0.02 0.25 ± 0.04 1.66* 1.05* 0.32
Isoleucine 0.14 ± 0.04 0.69 ± 0.08 0.36 ± 0.04 1.56 ± 0.25 0.54 ± 0.01 1.16 ± 0.05 2.26** 2.13** 1.10*
Valine 0.50 ± 0.11 1.54 ± 0.13 0.75 ± 0.08 3.00 ± 0.48 1.04 ± 0.04 2.19 ± 0.07 1.62** 1.99** 1.08*
Glycine 0.81 ± 0.09 1.80 ± 0.10 0.55 ± 0.06 2.62 ± 0.43 0.74 ± 0.05 1.88 ± 0.37 1.15** 2.25** 1.35*
Serine 0.16 ± 0.03 0.65 ± 0.07 0.79 ± 0.07 1.00 ± 0.07 0.91 ± 0.02 0.95 ± 0.05 2.00* 0.34 0.06
Alanine 1.56 ± 0.30 5.15 ± 0.74 2.90 ± 0.26 6.65 ± 0.81 4.64 ± 0.58 5.91 ± 0.56 1.72** 1.20** 0.35
Asparagine 0.03 ± 0.00 0.17 ± 0.04 0.07 ± 0.01 0.23 ± 0.02 0.19 ± 0.03 0.11 ± 0.01 2.49 1.63** −0.79
Fatty acids Arachidic acid 0.06 ± 0.01 0.10 ± 0.01 0.05 ± 0.00 0.07 ± 0.01 0.04 ± 0.00 0.04 ± 0.01 0.66* 0.38 −0.03
Lignoceric acid 0.03 ± 0.00 0.04 ± 0.01 0.02 ± 0.00 0.03 ± 0.00 0.02 ± 0.00 0.02 ± 0.00 0.56 0.74** −0.26
Behenic acid 0.03 ± 0.00 0.06 ± 0.00 0.03 ± 0.00 0.04 ± 0.00 0.03 ± 0.00 0.02 ± 0.00 0.88 0.48** −0.62*
Arachidonic acid 0.09 ± 0.01 0.10 ± 0.01 0.03 ± 0.00 0.06 ± 0.01 0.05 ± 0.00 0.04 ± 0.01 0.06 1.22** −0.26
Oleic acid 0.00 ± 0.00 0.03 ± 0.00 0.01 ± 0.00 0.04 ± 0.00 0.01 ± 0.00 0.03 ± 0.00 3.17** 1.90** 1.15**
Elaidic acid 0.09 ± 0.01 0.13 ± 0.04 0.05 ± 0.00 0.07 ± 0.01 0.06 ± 0.01 0.07 ± 0.01 0.46 0.69* 0.39
Cis-gondoic acid 0.00 ± 0.00 0.03 ± 0.00 0.01 ± 0.00 0.03 ± 0.00 0.01 ± 0.00 0.03 ± 0.00 3.24** 2.40** 2.00**
D-glyceric acid 0.36 ± 0.02 0.41 ± 0.03 0.20 ± 0.01 0.09 ± 0.01 0.20 ± 0.02 0.09 ± 0.03 0.19 −1.20* −1.23**
TCA Succinic acid 0.19 ± 0.02 0.10 ± 0.01 0.09 ± 0.00 0.04 ± 0.01 0.10 ± 0.01 0.03 ± 0.00 −0.94** −1.06** −1.74**
Citramalic acid 0.05 ± 0.00 0.03 ± 0.00 0.02 ± 0.00 0.01 ± 0.00 0.04 ± 0.01 0.01 ± 0.00 −0.52* −0.90** −2.43**
Glucose-1-phosphate 0.20 ± 0.02 0.10 ± 0.02 0.19 ± 0.00 0.07 ± 0.01 0.17 ± 0.00 0.07 ± 0.01 −1.09* −1.42** −1.29**
Fumaric acid 2.10 ± 0.21 7.74 ± 0.93 0.71 ± 0.10 0.57 ± 0.35 0.95 ± 0.08 0.62 ± 0.18 1.88** −0.32 −0.61
L-malic acid 26.68 ± 1.30 63.42 ± 6.58 11.16 ± 1.27 2.49 ± 0.52 13.45 ± 1.34 5.95 ± 1.85 1.25* −2.16** −1.18*
Citric acid 7.58 ± 1.00 29.38 ± 3.09 5.37 ± 1.19 7.40 ± 3.245 2.86 ± 0.41 3.85 ± 0.88 1.96** 0.46 0.43
Malonic acid 1.25 ± 0.18 3.93 ± 1.07 1.26 ± 0.22 1.11 ± 0.42 1.86 ± 0.22 0.49 ± 0.05 1.65* −0.18 −1.93*
Maleic acid 0.02 ± 0.01 0.04 ± 0.01 0.02 ± 0.00 0.01 ± 0.00 0.02 ± 0.00 0.01 ± 0.00 1.18 −0.63 −1.51**
Carbohydrates and polyols Mannitol 0.11 ± 0.02 0.03 ± 0.00 0.07 ± 0.01 0.02 ± 0.00 0.03 ± 0.00 0.01 ± 0.00 −1.83* −2.27** −1.80*
D-Arabitol 0.06 ± 0.00 0.04 ± 0.00 0.05 ± 0.00 0.03 ± 0.00 0.06 ± 0.01 0.02 ± 0.00 −0.54** −0.79** −1.79**
Dihydroxyacetone 0.05 ± 0.01 0.04 ± 0.01 0.03 ± 0.00 0.01 ± 0.00 0.05 ± 0.00 0.02 ± 0.00 −0.33 −1.27** −1.48**
Xylitol 2.27 ± 0.33 1.04 ± 0.09 0.94 ± 0.02 0.23 ± 0.05 1.05 ± 0.13 0.23 ± 0.05 −1.12* −2.02** −2.21**
Phytosphingosine 0.06 ± 0.00 0.03 ± 0.00 0.02 ± 0.00 0.01 ± 0.00 0.01 ± 0.00 0.01 ± 0.00 −0.85* −1.32** −0.04
Maltotriose 0.05 ± 0.00 0.05 ± 0.00 0.04 ± 0.00 0.01 ± 0.00 0.03 ± 0.01 0.01 ± 0.00 −0.16 −1.67** −1.56**
Tagatose 18.06 ± 0.69 3.83 ± 0.53 8.76 ± 0.19 2.31 ± 0.63 10.10 ± 1.40 0.61 ± 0.34 −2.24** −1.92** −4.04**
Galactose 0.20 ± 0.01 0.18 ± 0.01 0.20 ± 0.01 0.09 ± 0.03 0.39 ± 0.02 0.09 ± 0.01 −0.18 −1.10** −1.95**
Glucose 0.07 ± 0.01 0.02 ± 0.00 0.03 ± 0.00 0.01 ± 0.00 0.03 ± 0.00 0.01 ± 0.00 −1.79** −1.35** −1.81**
Fucose 0.24 ± 0.03 0.10 ± 0.02 0.10 ± 0.01 0.03 ± 0.00 0.13 ± 0.02 0.02 ± 0.00 −1.27** −1.67** −2.68**
Ribose 7.28 ± 0.69 3.07 ± 0.49 2.75 ± 0.09 1.60 ± 0.30 4.36 ± 0.45 0.90 ± 0.19 −1.04** −0.78** −2.28**
Mannose 19.20 ± 0.63 0.34 ± 0.11 9.35 ± 0.27 1.13 ± 0.44 8.40 ± 0.71 0.32 ± 0.20 −5.82** −3.04** −4.73**
Fructose 23.60 ± 0.45 5.14 ± 0.37 11.56 ± 0.46 2.90 ± 1.47 14.25 ± 1.87 0.86 ± 0.47 −2.20** −2.00** −4.05**
Sucrose 0.28 ± 0.09 0.33 ± 0.11 0.42 ± 0.32 0.20 ± 0.19 7.21 ± 0.57 0.05 ± 0.03 0.23 −1.03 −7.25**
Carboxylic acid 4-Aminobutyric acid 11.82 ± 0.96 3.47 ± 0.49 6.77 ± 0.49 2.00 ± 1.02 4.53 ± 0.22 1.21 ± 0.34 −1.77** −1.76** −1.90**
5-Aminovaleric acid 0.50 ± 0.07 0.34 ± 0.15 0.52 ± 0.07 0.08 ± 0.04 0.39 ± 0.12 0.06 ± 0.03 −0.55 −2.66** −2.72*
Galactonic acid 1.99 ± 0.53 1.43 ± 0.26 1.40 ± 0.21 0.56 ± 0.35 3.50 ± 0.37 0.32 ± 0.17 −0.48 −1.34 −3.47**
Saccharic acid 0.21 ± 0.02 0.16 ± 0.02 0.16 ± 0.00 0.06 ± 0.01 0.20 ± 0.02 0.05 ± 0.01 −0.44 −1.44** −1.94**
Threonic acid 12.03 ± 0.57 7.04 ± 0.31 4.75 ± 0.51 1.26 ± 0.13 5.80 ± 0.69 0.79 ± 0.13 −0.77* −1.91* −2.88**
4-Hydroxy-3-methoxybenzoic acid 0.03 ± 0.00 0.01 ± 0.00 0.05 ± 0.01 0.02 ± 0.00 0.06 ± 0.01 0.01 ± 0.00 −0.99** −1.50* −1.90**
Digalacturonic acid 0.03 ± 0.00 0.03 ± 0.00 0.02 ± 0.00 0.01 ± 0.00 0.01 ± 0.00 0.01 ± 0.00 0.05 −0.68* −0.01
Glycolic acid 0.16 ± 0.03 0.25 ± 0.07 0.06 ± 0.01 0.04 ± 0.00 0.06 ± 0.01 0.04 ± 0.00 0.64 −0.80** −0.54*
Gluconic acid 10.20 ± 1.17 36.88 ± 0.80 7.27 ± 0.72 12.53 ± 1.94 20.29 ± 1.05 6.10 ± 1.24 1.85** 0.78 −1.73**
Myristic Acid 0.05 ± 0.00 0.06 ± 0.01 0.04 ± 0.00 0.05 ± 0.01 0.03 ± 0.00 0.05 ± 0.00 0.38 0.58 0.55*
Nucleic acid Thymidine 0.01 ± 0.00 0.01 ± 0.00 0.01 ± 0.00 0.02 ± 0.00 0.00 ± 0.00 0.01 ± 0.00 0.23 1.49** 1.66**
Thymine 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.03 ± 0.00 0.01 ± 0.00 0.02 ± 0.00 −0.47 4.28** 1.15**
Uracil 0.52 ± 0.04 0.73 ± 0.06 0.48 ± 0.02 0.75 ± 0.03 0.51 ± 0.02 0.59 ± 0.04 0.48 0.66** 0.20
ROC Tricetin 0.02 ± 0.00 0.02 ± 0.00 0.01 ± 0.00 0.01 ± 0.00 0.01 ± 0.00 0.01 ± 0.00 0.11 0.00 −0.96**
5-Methoxytryptamine 0.00 ± 0.00 0.18 ± 0.10 0.06 ± 0.00 0.01 ± 0.00 0.10 ± 0.00 0.03 ± 0.00 5.92** −2.13** −1.54**
Sitosterol 0.02 ± 0.00 0.02 ± 0.00 0.01 ± 0.00 0.01 ± 0.00 0.02 ± 0.00 0.01 ± 0.00 −0.05 0.92** −0.67
Salicylic acid 0.02 ± 0.00 0.02 ± 0.00 0.01 ± 0.00 0.01 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.42* −0.63* −2.09**
Naringin 0.02 ± 0.00 0.02 ± 0.00 0.00 ± 0.00 0.02 ± 0.00 0.01 ± 0.00 0.00 ± 0.00 −0.07 3.93** −0.64**
Hydroxylamine 0.08 ± 0.00 0.05 ± 0.00 0.02 ± 0.00 0.02 ± 0.01 0.02 ± 0.01 0.01 ± 0.00 −0.80** 0.13 −0.71
Gallic acid 0.49 ± 0.05 0.58 ± 0.06 0.44 ± 0.02 0.38 ± 0.04 0.14 ± 0.01 0.33 ± 0.02 0.24 −0.21 1.19**
Fluorene 0.00 ± 0.00 0.06 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.02 ± 0.00 0.01 ± 0.01 4.29** 1.58* −1.15

The relative contents of metabolites are the means of data from four biological replicates using GC–MS. The fold changes was calculated using the formula log2(salt/control). Values were presented as the mean ± standard deviation of four biological replicates. CK, control treatment; NS, neutral salt stress; AS, alkaline salt stress. W, wild soybean; S, semi-wild soybean; M, cultivated soybean.

*

Significant difference at p < 0.05.

**

Significant difference at p < 0.01.