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. 2017 Jun 22;7:4033. doi: 10.1038/s41598-017-04330-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of LIPA as a GPI-anchored form. (A) Schematic view of the plasmid constructs of the His6-Flag (HF)-tagged secretory LIPA (sHF-LIPA) and the GPI anchored LIPA (sHF-LIPA-GPI). The signal sequence (SS) and GPI attachment signal of human CD59 were used. (B) Flow cytometric analysis of sHF-LIPA-GPI on the cell surface of P15 cells. The cells were stained with an anti-Flag antibody and a PE-conjugated goat anti-mouse IgG. After PI-PLC treatment, surface expression of sHF-LIPA-GPI was found to decrease.