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. 2017 Jun 22;7:4077. doi: 10.1038/s41598-017-04450-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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3D Bayesian cluster analysis of iPALM data of the distribution of LAT at the T cell immunological synapse. Representative cluster maps of LAT-mEos3.2 (a) in non-activated T cells, (b) in T cell synapses fixed after 4 mins, (c) in T cell synapses fixed after 8 mins. For each condition, (d) total number of localisations per ROI, (e) number of detected clusters per ROI, (f) percentage of localisations in clusters for each ROI, (g) cluster radii and (h) number of localisations per cluster. Bars represent mean values and S.E.M. ns = not significant, *p ≤ 0.01, **p ≤ 0.001, ***p ≤ 0.0001, Mann-Whitney U Test.