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. 2017 Jun 22;7:4077. doi: 10.1038/s41598-017-04450-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histograms of the z distribution of LAT at the T cell immunological synapse. (a) Average percentage of localisations in clusters at each z plane for the control condition. (b) Average percentage of localisations in clusters at each z plane for 4 minutes post-activation. (c) Average percentage of localisations in clusters at each z plane for 8 minutes post-activation. (d) Average fraction of number of clusters at each z position for the control condition. (e) Average fraction of number of clusters at each z position for 4 minutes post-activation. (f) Average fraction of number of clusters at each z position for 8 minutes post-activation. Histogram bins are 30 nm.