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. 2017 Jun 22;7:4066. doi: 10.1038/s41598-017-04403-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Serotype switching of mutants is confirmed by dot blotting of purified CPS preparations and specific antisera. (a) The CPS from a serotype 2 field strain reacts with anti-serotype 2 but not with anti-serotype 1 sera, while the CPS from mutant strain SS2to1/2 (W161C) reacts with both antisera (top panel). The CPS from a serotype 1/2 field strain reacts with both anti-serotype 1 and anti-serotype 2 sera, while the CPS from mutant strain SS1/2to2 (C161W) reacts with anti-serotype 2 but not with anti-serotype 1 sera (bottom panel). (b) The CPS from a serotype 14 field strain reacts strongly with anti-serotype 14 and weakly with anti-serotype 1 sera, while the CPS from mutant strain SS14to1 (W161C) reacts strongly with both antisera (top panel). The CPS from a serotype 1 field strain reacts strongly with both anti-serotype 14 and anti-serotype 1 sera, while the CPS from mutant strain SS1to14 (C161W) reacts strongly with anti-serotype 14 but weakly with anti-serotype 1 sera (bottom panel).