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. 2017 Jun 22;7:4066. doi: 10.1038/s41598-017-04403-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The antiphagocytic properties afforded to S. suis by CPS types 2, 1/2, 14, and 1 appear similar under in vitro conditions. Parental strains and isogenic mutants (1 × 10⁷ CFU/ml) were incubated for 60 min with J774 macrophages (multiplicity of infection = 100) in the presence of 50% murine serum. Results represent the mean (CFU/ml) + SEM of four independent experiments. Statistical analyses using the Student’s t-test showed no significant differences in the number of internalized bacteria between strains.