Table 6.
Effect of hyperoxia on the mRNA content of TNFα, TNFRI, and caspases in TIIcells
| number of cycles (Δct) | increase of mRNA | |||
| Control | Hyperoxia | ΔΔct | -fold | |
| TNFα | 3.66 ± 0.66 | 0.93 ± 0.30* | -2.73 | 6.6 |
| TNFRI | 5.02 ± 0.19 | 4.44 ± 0.11* | -0.58 | 1.5 |
| Caspase 8 | 10.3 ± 0.89 | 9.15 ± 0.35 | -1.15 | 2.2 |
| Caspase 3 | 5.33 ± 0.25 | 4.29 ± 0.48* | -1.04 | 2.1 |
The mRNA of three animals per group was isolated and combined. The content of specific mRNA was determined using Real time-PCR. In parallel, GAPDH was determined in each run as internal standard. The values are given as number of GAPDH-corrected cycle (Δct) ± SD of n = 3 determinations. ΔΔct is the difference of the number of cycles between control and hyperoxia; a decrease of Δct indicates an increase of the mRNA, also given as a factor of increase. Asterisk indicates a significant difference to control (p < 0.05).