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. 2017 Jun 22;7:4072. doi: 10.1038/s41598-017-04424-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SLC7A2 knockdown significantly impairs P. berghei intra-hepatic development. (a) Two different lines of Huh7 cells with stable knockdown of SLC7A2 were infected with luciferase-expressing P. berghei sporozoites and parasite load (luminescence) was assessed 48 h later. A scrambled shRNA sequence was used as a negative control. Error bars represent SEM. Pool of 4 independent experiments. (b) Quantification of the area of the EEFs in the 2 cell lines with SLC7A2 knockdown at 48 hpi by immunofluorescence microscopy. The knockdown efficiency of each shRNA sequence is indicated below each graph. Representative experiment out of 3 independent experiments. Both panels: Kruskal-Wallis with post-test Dunn’s. *p < 0.05 and ***p < 0.001.