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. 2017 Jun 22;7:4025. doi: 10.1038/s41598-017-04267-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of pLS/AMPV-C F&G construction. The open reading frame of the AMPV-C F gene, amplified from viral genomic RNA, was cloned into the pIRES-hrGFP-2a vector (pIRES, Clontech, Mountain View, CA) downstream of the IRES sequence. Subsequently, the IRES and the AMPV-C F ORF sequences were amplified and cloned into the pLS/AMPV-C G vector downstream of the NDV F ORF using an In-Fusion PCR Cloning Kit (Clontech). The NDV Gene Start and Gene End signal sequences and the AMPV-C F and G ORF sequences are underlined. The direction of the T7 promoter is indicated by a bold black arrow. HDVRz and T7Φ represent the site of the Hepatitis delta virus ribozyme and the T7 terminator sequences, respectively.