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. 2017 Jun 22;7:4025. doi: 10.1038/s41598-017-04267-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth dynamics of the recombinant viruses. DF-1 cells were infected with either rLS/AMPV-C F&G, rLS/AMPV-C G or LaSota strain at an MOI of 0.01. Every 12 h post-infection, virus was harvested by freeze-thaw cycles. Viral titers at each time point were determined by TCID₅₀ titration in DF-1 cells, in triplicates. The mean titer at each time point is expressed in log₁₀ TCID₅₀/ml.