Fig. 5.

Phylogenetic analysis of taxonomically uncharacterised metagenomic assemblies (unknowns) compared to the closest representative reference genomes. a Phylogenetic tree of “unknown” eukaryotic assemblies compared to reference Malassezia genomes. The inclusion of the Malassezia spp. and Ustilago maydis available reference genomes in the tree shows that unaffected and diseased skin is colonised by uncharacterised Malassezia and Malassezia spp. Marked with asterisk indicate the two Malassezia genomes reconstructed from the patient’s 102 left ear. Malassezia clusters, 1–3, are congruent with those reported previously,⁵⁴ with most of the Malassezia reconstructed genomes falling within cluster 2. b Phylogenetic tree of “unknown” bacterial assemblies in the Peptostreptococcaceae family. Anaerococcus spp. and Finegoldia magna reveal a novel Anaerococcus spp. on the ear of patient 9. c Phylogenetic tree of members of the Chromobacteriaceae (23 species) and Neisseriaceae (47 species) and “unknown” assemblies from Patients 9 and 101 are unable to be placed in either family