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. 2017 Jun 22;7:4092. doi: 10.1038/s41598-017-04460-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HUVECs and DRGs secreted a variety of proteins. (A) Map of cytokine array used to determine protein secretion from (B) HUVEC monocultures and (C) DRG-HUVEC co-cultures normalized to EGM-2 medium. To determine how the DRG population affected the secretion and consumption of proteins, (D) results from arrays assayed with DRG-HUVEC co-cultures were normalized to HUVEC monocultures. In all cases, red indicates overexpression of indicated proteins and blue indicates underexpression of indicated proteins, with gradations of each color indicating relative amount of over/under expression. POS and NEG wells are internal controls within the cytokine array assay. Data are compiled from two independent replicates.