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. 2017 Jun 22;7:4092. doi: 10.1038/s41598-017-04460-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of HUVEC secretion of BDNF. HUVECs were seeded at high (10,000 cell/cm²) or low (1,000 cell/cm²) densities and cultured in parallel with DRG-HUVEC co-cultures and assayed for BDNF using ELISA kits, and normalized to their respective seeding densities. Both concentrations of HUVECs secreted measurable amounts of BDNF significantly higher than EGM-2 and low density seeding of HUVECs produced higher concentrations of BDNF per cell than high density seeding of HUVECs. Data are presented as mean ± standard error. ***(p < 0.001) and brackets indicate significance from other treatments as determined by one-way ANOVA with Holm-Sidak post hoc analysis (Well sample size for each group shown in bars from 2 independent replicates).