Figure 2.

Determination of ROS and cell viability in SLC4A11 depleted human corneal endothelial cells. ROS induced by oxidative stress was analyzed by flow cytometry using H₂CFDA fluorescent probe. HCEnC were transfected with SLC4A11 targeted siRNA or control siRNA and exposed to 250 μM tBH, washed with 1X PBS, and ROS was determined (a). Cell viability was determined using MTT assay (b). Untreated cells were used as controls in each case. These experiments were performed twice.