Figure 6.

Overexpression of SLC4A11 in HEK 293 cells. Cells were transfected with wild-type. SLC4A11 plasmid or empty vectors and exposed to 250 µM of tBH. Cell viability (a), ROS generation (b) and Nitrotyrosine staining (c) were determined. For nitrotyrosine staining, cells were stained using anti-nitrotyrosine antibody, followed by Alexafluor 488 secondary antibody. Cells were imaged both under bright field and fluorescence and images were captured by Olympus IX73 using 20X objective. The experiments a, and b, were done three times in duplicates and experiment c was performed two times.