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. 2017 Jun 22;7:4020. doi: 10.1038/s41598-017-04164-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cellular morphologies associated with each glycan. (A) The cores had various levels of each antigen. The TMA contained two cores from each tumor, presented in pairs in the column graph. (B–G) The top two images in each group are the raw fluorescence from the second (left) and third (right) rounds of immunofluorescence. The red signal in the left image is CA19-9, and the red signal in the right image is sTRA. The lower images in each group are zoomed pictures corresponding to the white box. The lower left image is the H&E image overlaid with the detected signals. The signal from CA19-9 is orange, the signal from sTRA is cyan, and the overlapping signal is green. The lower right image is from the H&E stain. The asterisk in panel B marks vacuolated, invasive cells.